ADHDgene Database

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Large-scale studies

Data Summary

Published Variant
- SNP: 1391
- CNV: 398
- Others: 173
Published Gene: 359
Published Region: 128
Pathway by PBA: 8
Study: 361

Study Report

Basic Info

Reference	Ergul, E., 201221819229
Citation	Ergul, E., A. Sazci and I. Kara (2012). "Methylenetetrahydrofolate reductase gene polymorphisms in Turkish children with attention-deficit/hyperactivity disorder." Genet Test Mol Biomarkers 16(1): 67-69.
Study Design	case-control
Study Type	Candidate-gene association study
Sample Size	100 cases and 300 controls
Predominant Ethnicity	Caucasian
Population	Turkish
Gender	80 boys and 20 girls with ADHD, 240 boys and 60 girls of healthy controls
Age Group	Children/Adolescents : 5-12 years (mean age: 8.87; standard deviation [SD]=2.55) of cases, 5-12 years (mean age: 8.02; SD=2.69) of controls

Detail Info

Summary	The aim of the present study was to evaluate a possible association between 5,10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and ADHD. There is evidence to suggest that MTHFR C677T and A1298C polymorphisms alter the function of the enzyme, causing reduced folate and increased homocysteine levels in plasma. Two polymorphisms of the MTHFR gene, C677T (rs1801133) and A1298C (rs1801131), were analyzed in a sample of 100 Diagnostic and Statistical Manual of Mental Disorders-IV-diagnosed ADHD and 300 healthy controls using a polymerase chain reaction-restriction fragment length polymorphism method. We did not find any association between MTHFR 677T allele, MTHFR 1298C allele, and ADHD. In addition, there was no genotype association between the MTHFR gene and ADHD (chi(2)=1.711; df=2; p=0.425; chi(2)=2.946; df=2; p=0.229). Our data suggest that neither the MTHFR C677T polymorphism nor the MTHFR A1298C polymorphism was associated with ADHD in Turkish children. Thus, the MTHFR gene does not seem to play a role in the etiopathogenesis of ADHD in the cohort studied.
Total Sample	Subjects were consecutively recruited from the Istanbul University Hospital psychiatric clinic through the assessment of two child psychiatrists with the DSM-IV criteria. In all, 65% had the combined subtype, 25%the inattentive subtype, and 10%the hyperactiveimpulsive subtype of ADHD. All subjects were Caucasians of Turkish ancestry. The healthy controls were checked by two child psychiatrists for the ADHD symptoms and other neurological and behavioral disorders to make sure that all controls included were free from these disorders.
Sample Collection	Subjects were consecutively recruited from the Istanbul University Hospital psychiatric clinic through the assessment of two child psychiatrists with the DSM-IV criteria. The healthy controls were from the nursery of the Istanbul University Hospital.
Diagnosis Description	Subjects were consecutively recruited from the Istanbul University Hospital psychiatric clinic through the assessment of two child psychiatrists with the DSM-IV criteria. In all, 65% had the combined subtype, 25% the inattentive subtype, and 10% the hyperactiveimpulsive subtype of ADHD. Autism cases were excluded from the study. No other neurological or behavioral disorders were identified.
Technique	Genomic DNA was isolated from whole blood of ADHD patients and healthy controls using a salting out method as previously described. The MTHFR C677T and A1298C polymorphisms were genotyped using a polymerase chain reaction-restriction fragment length polymorphism method as previously described.
Analysis Method	Odds ratios, 95% confidence intervals, and chi-square analysis for a matched analysis were calculated using conditional logistic regression. Tests for independence and interaction between the MTHFR C677T and MTHFR A1298C polymorphisms were done using the likelihood ratio test. The Hardy-Weinberg equilibrium was verified for all tested populations. SPSS 12.0 for Windows (SPSS, Chicago, IL) was used for all of the statistical analyses. A P-value of < 0.05 was considered significant. The power analysis was conducted by post hoc power analysis.
Result Description	We did not find any association between MTHFR 677T allele, MTHFR 1298C allele, and ADHD. In addition, there was no genotype association between the MTHFR gene and ADHD (chi(2)=1.711; df=2; p=0.425; chi(2)=2.946; df=2; p=0.229). Our data suggest that neither the MTHFR C677T polymorphism nor the MTHFR A1298C polymorphism was associated with ADHD in Turkish children. Thus, the MTHFR gene does not seem to play a role in the etiopathogenesis of ADHD in the cohort studied.

SNPs reported by this study (count: 2)

SNP	Allele Change	Risk Allele	Statistical Values	Author Comments	Result of Statistical Analysis
rs1801131	A/C		allelic P-value=0.229, X²=2.946, df=2; genotypic P-value=0.555, X²=0.349, df=1, OR (95% CI)=0.869 (0.545-1.386) for AA; P-value=0.132, X²=2.264, df=1, OR (95% CI)=1.416 (0.899-2.229); for AC; P-value=0.183, X²=1.772, df=1, OR (95% CI)=0.614 (0.297-1.267) for CC	There were no significant allele or genotype differences bet...... There were no significant allele or genotype differences between cases and controls. More...	Non-significant
rs1801133	C/T		allelic P-value=0.425, X²=1.711, df=2; genotypic P-value=0.204, X²=1.613, df=1, OR (95% CI)=0.745 (0.473-1.174) for CC; P-value=0.351, X²=0.870, df=1, OR (95% CI)=1.242 (0.788-1.957) for CT; P-value=0.511, X²=0.432, df=1, OR (95% CI)=1.314 (0.581-2.991) for TT	There were no significant allele or genotype differences bet...... There were no significant allele or genotype differences between cases and controls. More...	Non-significant

Genes reported by this study (count: 1)