Study Report
Basic Info
Reference |
Renner, T. J., 201121739117
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Citation |
Renner T. J., Nguyen T. T., Romanos M., Walitza S., Roser C., Reif A., Schafer H., Warnke A., Gerlach M. and Lesch K. P. (2011) "No evidence for association between a functional promoter variant of the Norepinephrine Transporter gene SLC6A2 and ADHD in a family-based sample." Atten Defic Hyperact Disord, 3(3): 285-9.
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Study Design |
family-based |
Study Type |
Candidate-gene association study |
Sample Size |
235 children from 162 families |
Predominant Ethnicity |
Caucasian |
Population |
German |
Gender |
178 boys |
Age Group |
Children/Adolescents
:
The mean age of the affected children was 11.03 years (SD: 3.12 years).
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Detail Info
Summary |
Due to its influence on catecholaminergic signaling, variants of the coding gene (SLC6A2) have been widely investigated in ADHD. Several previous studies report an association between single nucleotide polymorphisms located in SLC6A2 and ADHD; however, the findings are inconsistent. The variant A-3081T (rs28386840) has been shown to have major influence on the expression levels of SLC6A2 due to sequence alteration at a repressor binding site, with the T-allele being associated with ADHD. We tested this potential association of A-3081T in a German family-based ADHD sample of 235 children from 162 families, which has a power >99% based on the previously reported odds ratios. There was no evidence for an overtransmission of the risk allele T (transmission rate: 48.5%, P = 0.55). |
Total Sample |
Two hundred and thirty-five children (178 boys) with ADHD from 162 families were recruited and phenotypically characterized by a team of experienced child and adolescent psychiatrists in the outpatient unit of the Department of Child and Adolescent Psychiatry and Psychotherapy, University of Wurzburg, according to DSM-IV criteria American Psychiatric Association. Families were included if they had one or more children affected with ADHD to perform family-based association and genome-wide linkage studies. The index patient was required to be older than 8 years and to fulfill DSM-IV criteria for the combined subtype, and further affected siblings had to be at least 6 years of age. In 107 families one child, in 40 families two, in 12 families three and in 3 families four affected children were recruited. |
Sample Collection |
Two hundred and thirty-five children (178 boys) with ADHD from 162 families were recruited and phenotypically characterized by a team of experienced child and adolescent psychiatrists in the outpatient unit of the Department of Child and Adolescent Psychiatry and Psychotherapy, University of Wurzburg. |
Diagnosis Description |
Two hundred and thirty-five children with ADHD from 162 families were recruited according to DSM-IV criteria American Psychiatric Association. |
Technique |
To genotype the variant A-3081T (rs28386840), polymerase chain reaction was performed as described by Kim et al. |
Analysis Method |
The two-sided pedigree disequilibrium test weighting on family size (PDT-sum) was performed to assess the transmission rates and to test for association. A nominal p-value lower than 0.05 was considered statistically significant. |
Result Description |
We tested this potential association of A-3081T in a German family-based ADHD sample of 235 children from 162 families, which has a power>99% based on the previously reported odds ratios. There was no evidence for an overtransmission of the risk allele T (transmission rate: 48.5%, P = 0.55). We conclude that A-3081T is not a major risk variant in our ADHD sample, though SLC6A2 remains an interesting candidate gene in ADHD, especially for the inattentive subtype. |
SNPs reported by this study (count: 1)
SNP |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
rs28386840 |
A/T |
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PDT P-value=0.5532 |
There was no evidence for an overtransmission of the risk T-......
There was no evidence for an overtransmission of the risk T-allele.
More...
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Non-significant
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Genes reported by this study (count: 1)
Gene |
Statistical Values/Author Comments |
Result of Statistical Analysis |
SLC6A2 |
We conclude that A-3081T is not a major risk variant in our ......
We conclude that A-3081T is not a major risk variant in our ADHD sample, though SLC6A2 remains an interesting candidate gene in ADHD, especially for the inattentive subtype.
More...
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Non-significant
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