Study Report
Basic Info
Reference |
Laurin N, 2008(c)17948899
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Citation |
Laurin N., Ickowicz A., Pathare T., Malone M., Tannock R., Schachar R., Kennedy J. L. and Barr C. L. (2008) "No evidence for genetic association between DARPP-32 (PP1R1B) polymorphisms and attention deficit hyperactivity disorder." Am J Med Genet B Neuropsychiatr Genet, 147(3): 339-42.
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Study Design |
family-based |
Study Type |
Candidate-gene association study |
Sample Size |
255 probands and 53 affected siblings |
Predominant Ethnicity |
Caucasian |
Population |
Canada |
Age Group |
Children/Adolescents
:
7-16 years
|
Detail Info
Summary |
Here, they tested the DARPP-32 gene, PPP1R1B, for association with ADHD using four polymorphic markers selected across the gene in a sample of 255 ADHD families. They did not detect evidence of association of individual marker alleles and haplotype analysis did not reveal significant association in this sample of families. Moreover, they found no relationship between the same alleles or haplotypes and symptom scores of inattention or hyperactivity/ impulsivity in these families using a quantitative approach. In conclusion, albeit a key regulatory role in dopamine signaling, their data do not support a major contribution of the DARPP-32 gene in ADHD. |
Total Sample |
255 probands and 53 affected siblings between 7 and 16 years old were included |
Diagnosis Description |
255 probands and 53 affected siblings between 7 and 16 years old were included if they met DSM-IV criteria for one of the three ADHD subtypes (predominantly inattentive, predominantly hyperactive/impulsive or combined). The methods of assessment, characteristics of the subjects, and inclusion/ exclusion criteria have been described previously, including the instruments used to collect information for the diagnosis of ADHD and co-morbid conditions [Quist et al., 2000; Laurin et al., 2005]. |
Technique |
DNA was extracted from blood lymphocytes using a high salt extraction method [Miller et al. 1988]. The markers were genotyped using Assay-on-demand SNP genotyping and the ABI 7900-HT Sequence Detection System. |
Analysis Method |
The TDTphase program from the UNPHASED package and the TRANSMIT program were used to test for biased transmission of alleles or haplotypes, respectively. Quantitative trait TDT analyses were carried out using the FBAT program v1.5.5, with the additive model of inheritance. |
Result Description |
They did not detect evidence of association of individual marker alleles and haplotype analysis did not reveal significant association in this sample of families. Moreover, they found no relationship between the same alleles or haplotypes and symptom scores of inattention or hyperactivity/ impulsivity in these families using a quantitative approach. |
SNPs reported by this study (count: 4)
SNP |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
rs1495099 |
G/C |
|
P-value=0.586, X2 (1 df)=0.297 in the categorical analysis; P-value>0.05 in the quantitative analysis |
no significant evidence of association
no significant evidence of association
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Non-significant
|
rs879606 |
G/A |
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P-value=0.442, X2 (1 df)=0.593 in the categorical analysis; P-value>0.05 in the quantitative analysis |
no significant evidence of association
no significant evidence of association
|
Non-significant
|
rs3764352 |
T/C |
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P-value=0.865, X2 (1 df)=0.029 in the categorical analysis; P-value>0.05 in the quantitative analysis |
no significant evidence of association
no significant evidence of association
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Non-significant
|
rs907094 |
A/G |
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P-value=0.933, X2 (1 df)=0.007 in the categorical analysis; P-value>0.05 in the quantitative analysis |
no significant evidence of association
no significant evidence of association
|
Non-significant
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Genes reported by this study (count: 1)
Gene |
Statistical Values/Author Comments |
Result of Statistical Analysis |
PPP1R1B |
no evidence of association of individual marker alleles and ......
no evidence of association of individual marker alleles and haplotypes
More...
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Non-significant
|