Study Report
Basic Info
Reference |
Simsek M, 200616545000
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Citation |
Simsek M., Al-Sharbati M., Al-Adawi S. and Lawatia K. (2006) "The VNTR polymorphism in the human dopamine transporter gene: improved detection and absence of association of VNTR alleles with attention-deficit hyperactivity disorder." Genet Test, 10(1): 31-4.
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Study Design |
case-control |
Study Type |
Candidate-gene association study |
Sample Size |
92 cases and 110 controls |
Predominant Ethnicity |
Omani |
Population |
Oman |
Age Group |
Children/Adolescents
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Detail Info
Summary |
The human dopamine transporter (DAT1) gene contains a variable number tandem repeat (VNTR) in its 3'- untranslated region because of repetition of a 40-bp core sequence. Methods available for the diagnosis of this polymorphism are limited in number. They have developed a new polymerase chain reaction (PCR) test, which is similar to that described originally by Vandenbergh's group, but provides a better detection of the VNTR alleles in the human DAT1 gene. Using two independent PCR methods, they have determined the distribution of VNTR alleles in 110 healthy Omani subjects, and in 92 children with attention-deficit hyperactivity disorder (ADHD). The frequency of the risk allele (DAT1*10) was similar in the healthy subjects and ADHD cases, indicating absence of association of this allele with ADHD in Oman. |
Total Sample |
This study included 110 healthy Omani blood donors at Sultan Qaboos University Hospital (SQUH) as control subjects, and 92 Omani children attending the child and adolescent psychiatry clinic at SQUH as ADHD cases. |
Sample Collection |
Omani |
Diagnosis Description |
The diagnosis of ADHD in children was according to the Diagnostic and Statistical Manual of Mental Disorders, Fourth edition (DSM-IV) criteria, which included a diagnostic questionnaire that was completed by the parents/guardians of each child in the presence of an attending child psychiatrist. |
Technique |
Five milliliters of ethylenediamine tetraacetic acid (EDTA)- blood were collected from each subject and genomic DNA was extracted from the frozen blood using a standard salting out procedure (Miller et al. 1988). The VNTR region of the DAT1 gene was amplified from the genomic DNA using two independent polymerase chain reactions (PCR) to minimize possible genotyping errors. Primers used in one PCR system were as described by Vandenbergh et al. (1992) (D1 forward, 5'-TGT GGT GTA GGG AAC GGC CTG AG-3' and D2 reverse, 5'- CTT CCT GGA GGT CAC GGC TCA AGG-3'). They used a new set of primers in the second PCR test (D3 forward: 5'-GCT CAG GCT ACT GCC ACT CAG GCA-3' and D4 reverse: 5'- GAT GTG GCA CGC ACC TGA GAG AAA-3'). More detailed description could be found in the original publication. |
Analysis Method |
statistical method was not mentioned in the original publication |
Result Description |
Using two independent PCR methods, they have determined the distribution of VNTR alleles in 110 healthy Omani subjects, and in 92 children with attention-deficit hyperactivity disorder (ADHD). The frequency of the risk allele (DAT1*10) was similar in the healthy subjects and ADHD cases, indicating absence of association of this allele with ADHD in Oman. |
Other variant reported by this study (count: 1)
Variant Name |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
SLC6A3 3'-UTR VNTR |
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P-value>0.05
P-value>0.05
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indicated absence of any strong association |
Non-significant
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Genes reported by this study (count: 1)
Gene |
Statistical Values/Author Comments |
Result of Statistical Analysis |
SLC6A3 |
indicated absence of any strong association between SLC6A3-V......
indicated absence of any strong association between SLC6A3-VNTR polymorphism and ADHD cases in Oman
More...
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Non-significant
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