Study Report
Basic Info
Reference |
Cho SC, 2010(b)20576502
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Citation |
Cho S. C., Kim H. W., Kim B. N., Kim J. W., Shin M. S., Cho D. Y., Chung S., Jung S. W., Yoo H. J., Chung I. W., Chung U. S. and Son J. W. (2010) "Neurotrophin-3 gene, intelligence, and selective attention deficit in a Korean sample with attention-deficit/hyperactivity disorder." Prog Neuropsychopharmacol Biol Psychiatry, 34(6): 1065-9.
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Study Design |
case-control and family-based |
Study Type |
Candidate-gene association study |
Sample Size |
202 cases, 159 controls, 151 trios |
Predominant Ethnicity |
Mongoloid |
Population |
Korea |
Gender |
174 ADHD subjects (86.1%) and 111 controls (69.8%) were boys |
Age Group |
Children/Adolescents
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mean age=9.0,SD=2.5
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Detail Info
Summary |
They investigated the relationships between ADHD and NTF3 gene polymorphism. They conducted a case-control analysis of 202 ADHD subjects and 159 controls, performed a transmission disequilibrium test (TDT) on 151 trios, and compared the intelligence quotient (IQ) and a continuous performance test (CPT) according to the genotype of two single-nucleotide polymorphisms (SNPs) (rs6332 and rs6489630) in the NTF3 gene. In the case-control and family-based analyses, NTF3 was not significantly associated with ADHD. However, in the ADHD probands, the subjects with AA genotype in the rs6332 SNP had significantly higher mean T-scores for commission errors on the CPT than did those with the AG genotypes (P-value=0.045). The mean IQ of the ADHD probands who had the CC genotype of the rs6489630 SNP were higher compared with those who had the CT or TT genotype (P-value=0.035). The mean T-score for response time on the CPT was higher in the subjects with TT genotype in the rs6489630 SNP compared to those with the CC or CT genotype, even after adjusting for the effect of IQ (P-value=0.021). These results provide preliminary evidence of an association between NTF3 and the intelligence and selective attention deficit in the Korean population. |
Total Sample |
The case-control analysis included 202 ADHD subjects with a mean age of 9.0(SD=2.5) years and 159 controls with a mean age 9.0(SD=2.7) years. The family-based analysis included 151 trios consisting of an affected subject and his or her biological father and mother. |
Sample Collection |
ethnically Korean |
Diagnosis Description |
All subjects with ADHD met the following inclusion criteria: (1) DSM-IV diagnosis of ADHD; (2) above the 90th percentile in the ADHD Rating Scale-IV (ARS); (3) T-scores greater than 60 on the attention problems profile of the Child Behavior Checklist (CBCL); and (4) intelligent quotient (IQ) higher than 71 on the Korean Educational Developmental Institute's Wechsler Intelligence Scale for Children (KEDIWISC). |
Technique |
The detection of a SNP was based on analysis of primer extension products generated from previously amplified genomic DNA using a chip-based MALDI-TOF mass spectrometry platform. All primers in the PCR and hME reactions were designed using Assay Designer 3.1. |
Analysis Method |
tested the family-based and case-control associations for each individual polymorphism and haplotype using the standard TDT method in Haploview or SNPAlyzer software version 7.0. Group differences in the clinical variables for the continuous data were computed using analysis of variance (ANOVA) or analysis of covariance (ANCOVA). When the comparison was significant, a post hoc Bonferroni test was performed to evaluate the differences between the groups. The analysis was conducted using SPSS statistical software (v. 15.0). |
Result Description |
In the case-control and family-based analyses, NTF3 was not significantly associated with ADHD. However, in the ADHD probands, the subjects with AA genotype in the rs6332 SNP had significantly higher mean T-scores for commission errors on the CPT than did those with the AG genotypes (P-value=0.045). The mean IQ of the ADHD probands who had the CC genotype of the rs6489630 SNP were higher compared with those who had the CT or TT genotype (P-value=0.035). The mean T-score for response time on the CPT was higher in the subjects with TT genotype in the rs6489630 SNP compared to those with the CC or CT genotype, even after adjusting for the effect of IQ (P-value=0.021). |
SNPs reported by this study (count: 2)
SNP |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
rs6332 |
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allelic P-value=0.774, X2 (1df)=0.082, genotypic P-value=0.938, X2 (2df)=0.129 in boys; allelic P-value=0.254, X2 (1df)=1.304, genotypic P-value=0.166 in girls; TDT P-value=0.801, X2 (1df)=0.06 in all; TDT P-value=0.469, X2 (1df)=0.53 in boys; TDT P-value=0.251, X2 (1df)=1.32 in girls |
no significant differences between the ADHD and control grou......
no significant differences between the ADHD and control groups in the genotype or allele frequencies and no preferential transmission from the TDT analysis
More...
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Non-significant
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rs6489630 |
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allelic P-value=0.855, X2 (1df)=0.033, genotypic P-value=0.747, X2 (2df)=0.583 in boys; allelic P-value=0.601, X2 (1df)=0.274, genotypic P-value=0.506 in girls; TDT P-value=0.930, X2 (1df)=0.01 in all; TDT P-value=0.926, X2 (1df)=0.01 in boys; TDT P-value=0.593, X2 (1df)=0.29 in girls |
no significant differences between the ADHD and control grou......
no significant differences between the ADHD and control groups in the genotype or allele frequencies and no preferential transmission from the TDT analysis
More...
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Non-significant
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Genes reported by this study (count: 1)
Gene |
Statistical Values/Author Comments |
Result of Statistical Analysis |
NTF3 |
no significant differences in the genotype frequencies and t......
no significant differences in the genotype frequencies and the haplotype analysis; in the case-control and family-based analyses, NTF3 was not significantly associated with ADHD
More...
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Non-significant
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