Study Report
Basic Info
Reference |
Li J, 200817918234
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Citation |
Li J., Wang Y., Hu S., Zhou R., Yu X., Wang B., Guan L., Yang L., Zhang F. and Faraone S. V. (2008) "The monoamine oxidase B gene exhibits significant association to ADHD." Am J Med Genet B Neuropsychiatr Genet, 147(3): 370-4.
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Study Design |
family-based |
Study Type |
Candidate-gene association study |
Sample Size |
548 ADHD probands |
Predominant Ethnicity |
Mongoloid |
Population |
China |
Age Group |
Children/Adolescents
:
mean age 10.0,SD=2.9 years
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Detail Info
Summary |
The current study sequenced the exons and the 5' and 3' flanking regions of the MAOB gene and found four common variants including 2276C>T and 2327C>T in exon 15, rs1799836 in intron 13 and rs1040399 in 3'-UTR. They assessed the association of these variants with ADHD in 548 trios collected from 468 males and 80 females probands. TDT analysis showed that alleles of each polymorphism were preferentially transmitted to probands (rs1799836, P-value=3.28E-15; rs1040399, P-value=1.87E-6; 2276T>C or 2327T>C, P-value=2.20E-6) and haplotype-based TDT analyses also found distorted transmission. In conclusion, this study provides the strongest evidence for the involvement of MAOB gene in the etiology of ADHD to date, at least in Han Chinese population. |
Total Sample |
548 ADHD probands were recruited from the ADHD outpatient clinic at the Child and Adolescent Psychiatry Division of the Sixth Hospital, Peking University in Beijing, PRC. |
Sample Collection |
Han Chinese descent |
Diagnosis Description |
All probands fulfilled DSM-IV diagnostic criteria for ADHD based on interviews by at least two different child psychiatrists with the aid of information from both biological parents and teachers. Of the 548 ADHD probands, 223 (40.7%) probands met criteria for ADHD combined type (ADHD-C), 30 (5.5%) met criteria for ADHD hyperactive-impulsive type (ADHD-HI) and 295 (53.8%) met criteria forADHDinattentive type (ADHD-I). |
Technique |
The promoter region, 15 exons and exon-intron junctions and partial sequences of 3' UTR were amplified by polymerase chain reaction (PCR) with primers given in the original publication. Variants of interest detected in this study were genotyped by restriction fragment length polymorphism (RFLP) in 548 ADHD trios. The method for genotyping these variants were given in Table II of the original publication. |
Analysis Method |
Linkage disequilibrium (LD) between variants was estimated by the EH program. The Transmission Disequilibrium Test (TDT) and the extended Transmission Disequilibrium Test (ETDT) were performed to test for distorted transmission of alleles or haplotypes. Holm's sequential Bonferroni procedure was used to correct for multiple testing. |
Result Description |
TDT analysis showed that alleles of each polymorphism were preferentially transmitted to probands (rs1799836, P-value=3.28E-15; rs1040399, P-value=1.87E-6; 2276T>C or 2327T>C, P-value=2.20E-6) and haplotype-based TDT analyses also found distorted transmission. |
SNPs reported by this study (count: 2)
SNP |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
rs1799836 |
A/G |
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TDT P-value=3.28E-15, X2=62.09 |
significantly biased transmitted to ADHD offspring
significantly biased transmitted to ADHD offspring
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Significant
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rs1040399 |
C/T |
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TDT P-value=1.87E-6, X2=22.72 |
significantly biased transmitted to ADHD offspring
significantly biased transmitted to ADHD offspring
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Significant
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Other variant reported by this study (count: 2)
Variant Name |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
MAOB exon15 2327T>C |
C/T |
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TDT P-value=2.20E-6, X2=20.65
TDT P-value=2.20E-6, X2=20.65
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significantly biased transmitted to ADHD offspring |
Significant
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MAOB exon15 2276T>C |
C/T |
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TDT P-value=2.20E-6, X2=20.65
TDT P-value=2.20E-6, X2=20.65
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significantly biased transmitted to ADHD offspring |
Significant
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Genes reported by this study (count: 1)
Gene |
Statistical Values/Author Comments |
Result of Statistical Analysis |
MAOB |
haplotype TCA P-value=0.003, X2=8.71, haplotype C......
haplotype TCA P-value=0.003, X2=8.71, haplotype CTA P-value=6.978E-19, X2=78.77, haplotype TCG P-value=1.620E-6, X2=23.00, haplotype CTG P-value=6.220E-11, X2=42.75, provides the strongest evidence for the involvement of MAOB gene
More...
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Significant
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