Study Report
Basic Info
Reference |
Li J, 2006(b)16563621
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Citation |
Li J., Wang Y., Zhou R., Wang B., Zhang H., Yang L. and Faraone S. V. (2006) "Association of attention-deficit/hyperactivity disorder with serotonin 4 receptor gene polymorphisms in Han Chinese subjects." Neurosci Lett, 401(1-2): 6-9.
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Study Design |
family-based |
Study Type |
Candidate-gene association study |
Sample Size |
326 nuclear families |
Predominant Ethnicity |
Mongoloid |
Population |
China |
Gender |
275 were male and 51 were female |
Age Group |
Children/Adolescents
:
mean age11.0,SD=2.3 years
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Detail Info
Summary |
To further investigate the role of other genes of the serotonergic pathway in ADHD, the current study examined variants of the serotonin 4 receptor gene in a relatively large sample of ADHD nuclear families. The T allele of the 83097 C> T polymorphism of HTR4 showed a tendency of preferential transmission to probands with ADHD. When haplotype TDT analysis of HTR4 was performed, they further found that the C/G haplotype of the 83097 C> T and 83198 A>G polymorphisms and the C/G/C haplotype of these and the -36 C> T polymorphism were under-transmitted to probands with ADHD. These results suggest that the HTR4 gene may play a role in the genetic predisposition to ADHD. |
Total Sample |
Three hundred and twenty six nuclear families participated in the present study. ADHD probands were recruited from the ADHD outpatient clinic at the Child and Adolescent Psychiatric Division of the Sixth Hospital, Peking University in Beijing, PRC. Among these probands, 275 were male and 51 were female. The average age was 11.0,SD=2.3 years and the average intelligence quotient (IQ) was 101.1,SD=13.5. |
Sample Collection |
Han Chinese |
Diagnosis Description |
All probands were interviewed by at least two different child psychiatrists with the aid of their biological parents and teachers, fulfilled DSM-IV diagnostic criteria for ADHD. One hundred and thirty-four (41.1%) probands met criteria for ADHD combined type (ADHD-C), 19 (5.8%) met criteria for ADHD hyperactive-impulsive type (ADHD-HI), and 173 (53.1%) met criteria forADHDinattentive type (ADHD-I). |
Technique |
Genomic DNA was extracted from blood using standard procedures. 83097C>T, 83198 A>G and -36T>C poly-morphisms were genotyped using the conditions described by OhtsUnited Kingdomi et al. Genotypes were read by two independent readers, and ambiguous or unidentifiable results were reamplified and rescored. Samples that continued to amplify poorly were eliminated from the study. |
Analysis Method |
The transmission disequilibrium test (TDT) and the extended transmission disequilibrium test (ETDT) were performed to test for evidence of distorted transmission of alleles and haplotypes, respectively. They used Holm's sequential Bonferroni method to correct for multiple testing. |
Result Description |
The T allele of the 83097C>T polymorphism of HTR4 showed a tendency of preferential transmission to probands with ADHD. When haplotype TDT analysis of HTR4 was performed, they further found that the C/G haplotype of the 83097C>T and 83198 A>G polymorphisms and the C/G/C haplotype of these and the -36 C>T polymorphism were under-transmitted to probands with ADHD. These results suggest that the HTR4 gene may play a role in the genetic predisposition to ADHD. |
Other variant reported by this study (count: 3)
Variant Name |
Allele Change |
Risk Allele |
Statistical Values |
Author Comments |
Result of Statistical Analysis |
HTR4 83097C>T |
C/T |
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TDT P-value=0.1, X2=2.699
TDT P-value=0.1, X2=2.699
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showed a tendency to be preferentially transmitted to ADHD probands |
Non-significant
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HTR4 83198A>G |
A/G |
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TDT P-value=0.823, X2=0.05
TDT P-value=0.823, X2=0.05
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was not statistically significant |
Non-significant
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HTR4 -36C>T |
C/T |
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TDT P-value=0.538, X2=0.38
TDT P-value=0.538, X2=0.38
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was not statistically significant |
Non-significant
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Genes reported by this study (count: 1)
Gene |
Statistical Values/Author Comments |
Result of Statistical Analysis |
HTR4 |
haplotype C/G from variants 83097 C>T/83198 A>G TDT P-value=......
haplotype C/G from variants 83097 C>T/83198 A>G TDT P-value=0.003, X2=8.783; haplotype C/G/C from 83097 C> T/83198 A>G/-36 C>T TDT P-value=0.016, X2=5.762; HTR4 gene may play a role in the genetic predisposition to ADHD
More...
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Significant
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